肾小管与集合管的重吸收课件.ppt

1、第二节第二节 肾小管与集合管的重吸收肾小管与集合管的重吸收 Reabsorption in the renal tubule and collecting duct中山大学基础医学院生理学教研室王庭槐 超滤液量超滤液量(ultrafiltrate volume)180L/日日 尿量尿量(urine volume)1.5L/日日 提示提示99%被重吸收被重吸收 1%排出体外排出体外 某物质某物质 终尿浓度终尿浓度u/血浆浓度血浆浓度P 反映物质被重吸收或分泌的情况反映物质被重吸收或分泌的情况 因为水的重吸收量达因为水的重吸收量达99%，流量缩小了，流量缩小了100倍，倍，所以所以u/p=100的

2、物质既不被重吸收又不被分泌和排的物质既不被重吸收又不被分泌和排泄泄 u/p100如肌酐如肌酐(creatinine)等，提示肾小管还有分泌等，提示肾小管还有分泌和排泄功能和排泄功能重吸收部位重吸收部位:近球小管近球小管(proximal tubule)14mm,刷状缘刷状缘(brush border)增大重吸收面积增大重吸收面积 髓袢髓袢(Henles loop)210mm 远球小管远球小管(distal tubule)13.6mm 集合管集合管(collecting duct)20mm一、重吸收的方式一、重吸收的方式1.被动重吸收被动重吸收(passive transport)：指小管液中的

3、水和溶指小管液中的水和溶质依借电化学差通过肾小管上皮细胞进入细胞质依借电化学差通过肾小管上皮细胞进入细胞外液的过程。外液的过程。Water and solutes are transported across the tubular epithelial cells into the extracellular fluid that is mediated by electronic and chemical forces.水水借渗透压借渗透压(osmotic forces)之差被动重吸收，渗之差被动重吸收，渗透压差是其动力。透压差是其动力。溶质溶质浓度差浓度差(difference of c

4、oncentration)和电位差和电位差(difference of potential)（电化学差）是其被动重吸收（电化学差）是其被动重吸收的动力。的动力。浓度差浓度差 电位差电位差 Na+Na+主动主动 Na+Na+H2O Cl-Cl _ 尿素尿素 尿素被动扩散尿素被动扩散 特特点点：顺顺电电化化学学差差进进行行，不不需需耗耗能能 2.主动重吸收主动重吸收(active transport)：指肾小管上皮指肾小管上皮细胞逆电化学差，将小管内溶质主动转细胞逆电化学差，将小管内溶质主动转运到小管外组织间液的过程。运到小管外组织间液的过程。Active transport can move a

5、 solute against an electrochemical gradient and requires energy derived from metabolism.逆浓度差逆浓度差 逆电位差逆电位差 髓袢升支粗段髓袢升支粗段(thick segment of ascending limb)继继发性主动转运发性主动转运(secondary active transport)特点：逆电化学差特点：逆电化学差 耗能耗能 Characteristics:against an electrochemical gradient and requires energy)葡萄糖葡萄糖(glucos

6、e)、氨基酸氨基酸(amino acid)、Na+等主等主动重吸收动重吸收二、几种物质的重吸收二、几种物质的重吸收1.Na+的重吸收：的重吸收：方式方式主动重吸收主动重吸收(active transport)原尿原尿(glomerular filtrate)Na+500g/日，日，终尿终尿(urine)Na+35g/日日 99%回收回收 地点和吸收比例地点和吸收比例：近球小管：占滤过量近球小管：占滤过量6570%远曲小管：远曲小管：10%髓袢：髓袢：20%集合管：耗集合管：耗O2 1克，重吸收克，重吸收Na+2030克克主动转运主动转运各段小管重吸收各段小管重吸收Na+的机制的机制近球小管近球

7、小管Proximal tubule 近球小管前半段：近球小管前半段：Na+与与GS、氨基酸同向转运氨基酸同向转运(symport)主动重吸收主动重吸收(active transport)Na+与与H+逆向转运逆向转运(antiport)近球小管后半段：近球小管后半段：Na+、Cl-细胞旁路被动重吸收细胞旁路被动重吸收(paracellular pathway)。近球小管近球小管泵漏模式泵漏模式(pump-leak model)A 首先小管腔首先小管腔Na+顺浓度差扩散入小管细胞内顺浓度差扩散入小管细胞内There is a concentration gradient favoring sod

8、ium diffusion into the cell.B 细胞侧膜的钠泵将其泵入细胞间隙细胞侧膜的钠泵将其泵入细胞间隙The cell has sodium pump to transport sodium out of the cell into the interstitium.C 水因渗透压被吸引到间隙，造成间隙内静水水因渗透压被吸引到间隙，造成间隙内静水压升高压升高Water moves to the interstitium by osmosis,and it leads to a high level of hydrostatic pressure in interstitium

9、.D 静水压升高引起静水压升高引起Na+和水通过基膜进入细胞和水通过基膜进入细胞间液和相邻毛细血管，并有回漏现象间液和相邻毛细血管，并有回漏现象.Sodium and water are reabsorbed from the interstitial fluid into the peritubular capillaries by hydrostatic pressure,meanwhile,there also exits leakage from interstitial fluid to tubule.E 在在Na+被重吸收时，尚有相当量的负离子被重吸收时，尚有相当量的负离子（HCO

10、3-或或ClCl-）顺顺Na+被重吸收时造成的被重吸收时造成的电位差而被重吸收。电位差而被重吸收。Many negative ions are reabsorbed by difference of potential induced by the process of sodium reabsorption.远曲小管：远曲小管：Na+重吸收量少重吸收量少A 紧密连接紧密连接(tight junctions)对对Na+通透性低，回漏通透性低，回漏量少量少B 管内外浓度差大，电位差大管内外浓度差大，电位差大 20mfT/L:140mfT/L 管内管内-1045 mVC 管腔膜管腔膜(lumina

11、l membrane)和管周膜和管周膜(basolateral membrane)分布有分布有Na+泵泵(sodium pump)，可将可将Na+泵泵到细胞外液和毛细血管内到细胞外液和毛细血管内D 伴有负离子重吸收和伴有负离子重吸收和Na+-H+、Na+-K+交换交换 髓袢髓袢(Henles loop)：主动转运与：主动转运与ClCl-继发继发性主动转运性主动转运(secondary active transport)升支粗升支粗段被动转运相结合。段被动转运相结合。集合管集合管(connecting tubule)：主动转运，主动转运，Na+重吸收造成管内重吸收造成管内-35-35mVmV。机

12、制未明机制未明2.ClCl-的重吸收：的重吸收：大部分是伴随大部分是伴随Na+的主动重吸收而被动重吸收。的主动重吸收而被动重吸收。When sodium is reabsorbed through the tubular epithelial cell,negative ions such as chloride are transported passively along with sodium.各段小管重吸收各段小管重吸收ClCl-的机制：的机制：近球小管近球小管(proximal tubule)：管内负电位是管内负电位是Na+依赖性依赖性 A Na+的主动重吸收形成小管内外电位差，管的

13、主动重吸收形成小管内外电位差，管内内-4-4mVmV Sodium is positively reabsorbed from the tubule.This creates a difference of potential.B 负离子负离子HCO3-、ClCl-顺电位差被动重吸收顺电位差被动重吸收 Negative ions such as HCO3-、ClCl-are passively reabsorbed by difference of potential.C HCO3-比比ClCl-优先重吸收，加上渗透压差导优先重吸收，加上渗透压差导致水的重吸收致水的重吸收 小管液中小管液中Cl

14、Cl-浓度浓度 Preferential reabsorption of HCO3-and the reabsorption of water by osmosis pressure lead to a high concentration of chloride in tubule.D ClCl-的管内外浓度差又造成的管内外浓度差又造成ClCl-被动重吸收被动重吸收 Cl-is reabsorbed passively by the difference of concentration.髓袢升支粗段，管腔内正电位（髓袢升支粗段，管腔内正电位（+2+2+10+10mVmV）正电位依赖正电位依

15、赖ClCl-、K K+存在。存在。NaNa+、ClCl-、K K+由同一载体协同转运。由同一载体协同转运。Movement of sodium across the luminal membrane is mediated primarily by a 1-sodium,2-chloride,1-potassium cotransporter.用哇巴因用哇巴因(-)(-)钠泵，转运受阻，提示钠泵，转运受阻，提示NaNa+泵泵参与此过程，机制可能如下：参与此过程，机制可能如下：The transport is blocked by using ouabain which blocks the a

16、ctivity of sodium pump.A 升支粗段上皮细胞管周膜的升支粗段上皮细胞管周膜的NaNa+泵将泵将NaNa+由胞内泵向组织间液由胞内泵向组织间液 Sodium is transported from the epithelial cell into interstitial fluid by the activity of sodium pump in the epithelial cell basolateral membranes.B NaNa+被泵出后，细胞内被泵出后，细胞内NaNa+，出现管出现管腔内与细胞内腔内与细胞内NaNa+浓度差浓度差 Sodium pump

17、maintains a low intracellular sodium concentration.C C 管腔内管腔内NaNa+顺此浓度差扩散到细胞顺此浓度差扩散到细胞内，但须与内，但须与ClCl-、K K+由同一载体协同由同一载体协同转运。转运。NaNa+:2Cl:2Cl-:K:K+The low intracellular sodium concentration in turn provides a favorable gradient for movement of sodium from the tubular fluid into the cell.In the thick a

18、scending loop,movement of sodium across the luminal membrane is mediated by a 1-sodium,2-chloride,1-potassium cotransporter.D 进入细胞后，进入细胞后，Na+经经Na+泵泵到组织间液泵泵到组织间液 Na+is transported to interstitial fluid by sodium pump.Cl-顺浓度差经管周膜扩散到组织间液顺浓度差经管周膜扩散到组织间液 Cl-diffuses into interstitial fluid through basola

19、teral membrane.K+由于浓度差经管腔膜返回管腔内由于浓度差经管腔膜返回管腔内 K+returns to tubule through basolateral membrane by difference of concentration.E 由于由于Cl-进入组织间液多，进入组织间液多，K+返回管腔返回管腔内液多，造成管腔内正电位内液多，造成管腔内正电位 特点：特点：Na+主动转运主动转运(active transport)，Cl-继继发性主动转运发性主动转运(secondary active transport)速尿速尿(furosedon)、利尿酸、利尿酸(-)(crinur

20、yl)3.3.水的重吸收：水的重吸收：99%99%重吸收，重吸收，1%1%排出体外排出体外 水的重吸收：水的重吸收：在近球小管重吸收，伴溶质而重吸收，与体内是在近球小管重吸收，伴溶质而重吸收，与体内是否缺水无关否缺水无关In proximal tubule,the reabsorption of water is accompanied with the reabsorption of solutes.在远曲小管和集合管吸收，吸收量受调节，体内在远曲小管和集合管吸收，吸收量受调节，体内缺少水时重吸收多，体内不缺水时重吸收少。缺少水时重吸收多，体内不缺水时重吸收少。In distal tubul

21、e and collecting duct water reabsorption is regulated by the condition of body.近球小管近球小管656570%70%水在各段小管水在各段小管 髓袢髓袢 10%10%重吸收比例重吸收比例 远曲小管远曲小管 10%10%集合管集合管 10 1020%20%近球小管管壁对水通透性高，高远曲小管近球小管管壁对水通透性高，高远曲小管34倍，倍，是一种等渗重吸收。是一种等渗重吸收。In proximal tubule，the process of water reabsorption is isosmotic because t

22、he permeability of tubule to water is high.髓袢、远曲小管集合管对水的重吸收机制，见尿液髓袢、远曲小管集合管对水的重吸收机制，见尿液浓缩与稀释。浓缩与稀释。4.HCO3-的的重吸收重吸收 特点：肾小管重吸收特点：肾小管重吸收HCO3-是以是以CO2的形式而非直接以的形式而非直接以HCO3-的形式进行，回到血中的的形式进行，回到血中的HCO3-是由细胞产生，是由细胞产生，并非小管液中的并非小管液中的HCO3-。HCO3-is reabsorbed as a gas form.HCO3-比比Cl-优先重吸收，是因为优先重吸收，是因为CO2能迅速透过管腔膜。

23、能迅速透过管腔膜。5.K+的重吸收：的重吸收：滤过液滤过液35克克/日，终尿日，终尿24克克/日日 主细胞重吸收主细胞重吸收Na+和水和水,分泌分泌K+。闰细胞则主要分泌。闰细胞则主要分泌H+。The principal cells reabsorb sodium and water and secrete potassium;the intercalated cells secrete hydrogen.特点：滤过液中的特点：滤过液中的K+绝大部分被重吸收（在近球小管），绝大部分被重吸收（在近球小管），终尿中的终尿中的K+主要是由远曲小管和集合管分泌。主要是由远曲小管和集合管分泌。Almos

24、t all potassium in proximal tubule is reabsorbed,and the potassium in urine is mostly secreted by distal tubule and collecting duct.近球小管管腔内近球小管管腔内-4-4mVmV，K K+重吸收逆电位差主动重吸收。重吸收逆电位差主动重吸收。6.6.葡萄糖的重吸收：葡萄糖的重吸收：A 逆浓度差主动重吸收逆浓度差主动重吸收B 借助借助Na+的主动重吸收而继发主动重吸收的主动重吸收而继发主动重吸收C 与刷状缘载体蛋白有关，协同转运与刷状缘载体蛋白有关，协同转运D 胞内葡萄

25、糖通过管周膜进入组织间液的过程是易化扩散胞内葡萄糖通过管周膜进入组织间液的过程是易化扩散。A specific carrier protein in the brush border combines with a sodium ion and a glucose molecule at the same time.The transport mechanism is so efficient that it removes virtually all the glucose from the tubular lumen.After entry into the cell,glucose ex

26、its across the basolateral membranes by facilitated diffusion,driven by the high glucose concentration in the cell.肾糖阈肾糖阈(renal glucose threshold)：当血中葡萄糖浓度：当血中葡萄糖浓度160160180180mg%mg%时，超过部分肾小管重吸收葡萄糖的能力，出现时，超过部分肾小管重吸收葡萄糖的能力，出现尿糖尿糖。When the concentration of glucose in blood is above 160180mg%,a small a

27、mount of glucose begins to appear in the urine.葡萄糖吸收极限量葡萄糖吸收极限量(transport maximum)：当血糖浓度增当血糖浓度增高到令全部肾小管对糖重吸收的能力到达极限时，此值高到令全部肾小管对糖重吸收的能力到达极限时，此值叫。（男叫。（男375375mg/minmg/min，女女300300mg/minmg/min）The overall transport maximum for the kidneys is reached when all nephrons have reached their maximal capacit

28、y to reabsorb glucose.E 如近球小管对如近球小管对Na+重吸收重吸收 ，葡萄糖吸收极限量也，葡萄糖吸收极限量也 。7.其它物质重吸收：其它物质重吸收：氨基酸氨基酸同葡萄糖同葡萄糖 HPO4、SO4与与NaNa+伴联伴联第三节第三节 肾小管与集合管肾小管与集合管的分泌和排泄的分泌和排泄 肾小管分泌肾小管分泌(tubular secretion)：指小管上皮细胞指小管上皮细胞通过新陈代谢，将它所产生的物质分泌通过新陈代谢，将它所产生的物质分泌到小管液中去的过程。到小管液中去的过程。肾小管排泄肾小管排泄(tubular excretion)：指肾小管上皮：指肾小管上皮细胞将血液

29、中某些物质直接排到小管中细胞将血液中某些物质直接排到小管中去的过程。去的过程。1.H+的分泌的分泌 H+的产生：的产生：CO2+H2O H2CO3 HCO3-+H+H+-Na-Na+交换，方向相反交换，方向相反,叫逆向交换叫逆向交换(counter-transport)H+分泌到小管液中，主要在近球小管发分泌到小管液中，主要在近球小管发生。生。远曲小管和集合管：除远曲小管和集合管：除H+-Na-Na+交换外，还交换外，还有有K+-Na-Na+交换，两者相互抑制，当酸中毒交换，两者相互抑制，当酸中毒时时H+，H+-Na-Na+交换加强，交换加强，K+-Na-Na+交换交换 ，或造成血或造成血K+

30、，反过来用乙酰唑胺反过来用乙酰唑胺(-)碳酸酐酶碳酸酐酶(carbonic anhydrase)，使，使H+以纠正酸中以纠正酸中毒，会使毒，会使H+-Na-Na+交换交换 ，K+-Na-Na+交换交换 ，造，造成血成血K+。（临床上应予注意）（临床上应予注意）2.NH3的分泌的分泌 远球小管和集合管的上皮细胞在代谢过程中产远球小管和集合管的上皮细胞在代谢过程中产生生NH3 The epithelial cells of the distal tubule and collecting duct produce NH3 during the process of metabolism.谷氨酰胺谷

31、氨酰胺（Glutamyl)(脱氢）脱氢）NH3 扩散到小扩散到小管液管液 NH3+H+NH4+3K+的分泌的分泌来源：尿来源：尿K+来自远曲小管和集合管的分泌，原尿中的来自远曲小管和集合管的分泌，原尿中的K+在近球小管已被重吸收入血。在近球小管已被重吸收入血。The potassium ions in urine is secreted by distal tubule and collecting duct,and the potassium ions in ultrafiltrate is reabsorbed into blood in proximal tubule.特点：特点：A.K

32、+的分泌是一种被动分泌过程，与的分泌是一种被动分泌过程，与Na+-K+交换交换有关。有关。B.Na+主动重吸收，造成管腔内主动重吸收，造成管腔内-10-45mV，促使促使K+从组织液扩散入管腔内液。从组织液扩散入管腔内液。4.其它物质排泄：其它物质排泄：肌 酐肌 酐（c r e a t i n i n e）、对 氨 基 马 尿 酸、对 氨 基 马 尿 酸（aminohippurate）既滤过又排泄既滤过又排泄 青 霉 素青 霉 素(b e n z y l p e n i c i l l i n）、酚 红、酚 红(phenolsulfonphthalein）主要是排泄主要是排泄5.影响肾小管与集

33、合管泌尿机能的因影响肾小管与集合管泌尿机能的因素素(1)小管液中溶质的浓度小管液中溶质的浓度(the concentration of solute in tubule)溶质浓度溶质浓度 ，渗透压，渗透压 ，重吸收，重吸收 。渗透性利尿渗透性利尿(osmotic diuresis)提高小管液提高小管液溶质浓度，达到利尿溶质浓度，达到利尿(2)肾小球滤过率肾小球滤过率(glomerular filtration rate)球管平衡球管平衡(glomerulotubular balance)：在近球小管中：在近球小管中：GFR ，重吸收率重吸收率 ；反之亦然反之亦然 即不管即不管GFR升高或降低，

34、滤液的重吸收升高或降低，滤液的重吸收率始终占率始终占GFR的的6570%左右（重吸收左右（重吸收率为率为6570%）One of the most basic mechanisms for controlling tubular reabsorption is the intrinsic ability of the tubules to increase their reabsorption rate in response to increased tubular load.This phenomenon is referred to as glomerulotubular balance

35、.If GFR is increased,the absolute rate of proximal tubular reabsorption also increases,and the percentage of GFR reabsorbed in the proximal tubular remains relatively constant at about 65-70 percent.机制：机制：a.近球小管对近球小管对Na+的定比重吸收，对的定比重吸收，对Na+重吸收量重吸收量是滤过量的是滤过量的6570%.The percentage of GFR reabsorbed in t

36、he proximal tubular remains relatively constant at about 65-70 percent.b.肾小管重吸收机能对肾小管重吸收机能对GFR的影响。的影响。重吸收重吸收 小管内压小管内压 囊内压囊内压 有效滤过压有效滤过压 滤过滤过 球管平衡在渗透性利尿球管平衡在渗透性利尿(osmotic diuresis)时会被打乱，时会被打乱，6570%水水肿肿第四节第四节 尿液的浓缩和稀释尿液的浓缩和稀释 concentration and dilution of urine 肾脏对尿液的浓缩和肾脏对尿液的浓缩和稀释能力在调节水平衡方稀释能力在调节水平衡方

37、面有极为重要的作用。面有极为重要的作用。一、尿浓缩和稀释的机一、尿浓缩和稀释的机制制逆流学说逆流学说（一）逆流倍增与逆流交换（一）逆流倍增与逆流交换 髓袢、集合管结构排列相髓袢、集合管结构排列相似于逆流倍增的模型似于逆流倍增的模型 直小血管的结构排列近似直小血管的结构排列近似于逆流交换模型。于逆流交换模型。（二）逆流学说（二）逆流学说 用冰点降低法测定肾分层切用冰点降低法测定肾分层切片的渗透压，发现：皮质部片的渗透压，发现：皮质部组织液体与血浆是等渗的，组织液体与血浆是等渗的，髓质部随髓质外层向乳头部髓质部随髓质外层向乳头部深入而逐渐升高，即渗透压深入而逐渐升高，即渗透压由外向内逐步升高，有明

38、确由外向内逐步升高，有明确梯度。梯度。1.髓质渗透压梯度形成机制髓质渗透压梯度形成机制the major factors that contribute to the buildup of solutes concentration into the renal medulla are as follows:A.外髓部渗透压梯度主要是由外髓部渗透压梯度主要是由髓袢升支粗段髓袢升支粗段NaCl的重吸收形成的（的重吸收形成的（Na+主动重吸收，主动重吸收，Cl-继继发性主动重吸收）发性主动重吸收）The main force is active transport of sodium ions a

39、nd co-transport of potassium,chloride,and other ions out of the thick portion of the ascending limb of the loop of Henle into the medullary interstitium.B.内髓部组织间液的渗透压是内髓部组织间液的渗透压是由由内髓部集合管内髓部集合管扩散出来的尿素以及扩散出来的尿素以及升支细段升支细段扩散出来的扩散出来的NaCl这两个因素形成这两个因素形成.Passive diffusion of large amounts of urea from the

40、inner medullary collecting ducts into the medullary interstitium and NaCl from thin segment of ascending limb.C.髓袢升支粗段对髓袢升支粗段对Na+和和Cl-的主动重吸的主动重吸收是主要动力，收是主要动力，尿素再循环尿素再循环则促成了整则促成了整个髓质渗透压梯度的建立。个髓质渗透压梯度的建立。The main force is the active transport of sodium ions and chloride ions in thick portion of the as

41、cending limb of the loop of Henle,and urea recirculation also contributes to the whole hyperosmotic renal medullary interstitium.1.髓质渗透压梯度形成机制髓质渗透压梯度形成机制 D.远曲小管及皮质部和外髓部的集合管对尿素不易通透,水被重吸收,小管液中尿素的浓度逐渐升高。In distal tubule and collecting duct,there is little permeability to urea,and water is reabsorbed,so

42、 that the concentration of urea rises gradually.E.髓袢降支细段对尿素及Na+都不易通透,对水易通透,水被“抽吸”出来,小管液被浓缩，其中尿素及Na+的浓度不断升高。Becasuse there is a high permeability to water and little permeability to urea and sodium ions in thin segment of descending limb,water is suctioned by osmosis and the tubular fluid reaches equ

43、ilibrium with the surrounding interstitial fluid of the renal medulla,which is very hypertonic.*形成机制简图*（三）尿液的浓缩与稀释（三）尿液的浓缩与稀释 1.尿液浓缩：机体缺水尿液浓缩：机体缺水血浆晶体渗透血浆晶体渗透压压 ADH 集合管对水通透性集合管对水通透性 ，管外为高渗环境，管内水份被吸到管管外为高渗环境，管内水份被吸到管外外小管液浓缩小管液浓缩When body is in lack of water,the crystal osmotic pressure increases,and

44、this promotes the release of ADH,thus,the permeability to water in collecting duct increases,the water in tubule is reabsorbed into the interstitial,so it forms a concentrated urine.2.尿液稀释：机体水过剩尿液稀释：机体水过剩血浆晶体渗透压血浆晶体渗透压 ADH 集合管对水不通透。因而不受髓质高集合管对水不通透。因而不受髓质高渗压影响，加上流经远曲小管的渗压影响，加上流经远曲小管的Na+继续被主动重吸收，继续被主动

45、重吸收，水却难于吸收，小管液渗透压水却难于吸收，小管液渗透压 形成低渗尿排出。形成低渗尿排出。When there is a large excess of water in the body,the crystal osmotic pressure will decrease,and this reduces the release of ADH,so the collecting duct is impermeable to water,and the sodium ion is reabsorbed in distal tubule,all these lead to the tubular osmosis pressure decreasing and forming a dilute urine.(四四)、直小血管的逆流交换直小血管的逆流交换作用作用 保持肾髓质的高渗作用，保持肾髓质的高渗作用，并将水，包括集合管和并将水，包括集合管和髓袢降支重吸收的水带髓袢降支重吸收的水带走。走。Countercurrent exchange in the vasa recta preserves hyperosmolarity of the renal medulla.