简介Waters-UPLC故障排除课件.ppt

1、2013 Waters Corporation 12023-1-312013 Waters Corporation 2Waters UPLC Troubleshooting Training 2023-1-322013 Waters Corporation 3ACQUITY UPLC Family：UPLC&H-ClassWaters UPLC SystemUPLC Solvent Management (BSM)UPLC Sample Management (SM)Waters ACQUITY H-Class SystemQuaternary Solvent Manager(QSM)Samp

2、le Manager-Flow Through Needle(SM-FTN)Waters UPLC Detector Troubleshooting TrainingWaters UPLC PDA and PDAeTUVFLRELSDOpen Discussion2023-1-332013 Waters Corporation 42023-1-342013 Waters Corporation 52023-1-352013 Waters Corporation 6Detectors:Optical and/or Mass SpecTunable UV or Photodiode ArrayOp

3、timized flow cell for UPLCHigh speed data samplingColumn Manager:Innovative pivot designPositions column to detectorE-Cord connectionSample Manager:Low dispersion XYZZ FormatFast cycle timesLow carryoverPlates and/or vialsOptional Sample OrganizerBinary Solvent Manager:High pressure blendingBinary g

4、radientsFour solvent choicesOn-line degassingLow dispersion designUPLC pressure capabilitiesSample Organizer:(option)Expands capacity(22/15/8)Shuttles plate feedHeated/chilledSystem Considerations:Small FootprintRedesigned tubing and fittingsConsolidated waste managementIntegrated system diagnostics

5、Connections Insight remote diagnostics2023-1-362013 Waters Corporation 7Waste Tubing management Chromatographic solvents:oA1/A2,B1/B2Two Sample Manager wash solventsSeal Wash Common fittings and tools for usersColumn inlets/outlets and fittings oFinger tight,turn(use 1/4”wrench)Dedicated tubing3 pie

6、ce compression screw and ferrule Small footprint 13”wide24.25”with Sample Organizer2023-1-372013 Waters Corporation 82023-1-382013 Waters Corporation 9 15 K psi performance Accurate and precise flow rates Accurate and precise composition Low delay volume for gradient applicationso5%Ethyl AcetateTolu

7、ene Chlorinated solvents:(Trichlorobenzene)2023-1-3142013 Waters Corporation 152023-1-3152013 Waters Corporation 16 Pressure Transducer Valve Manifold Drive Gears Wash SyringesWeak Wash Strong WashMetering Syringe Drive Assembly 2023-1-3162013 Waters Corporation 17 Metering Syringe Valve3 Way Rotary

8、 Valve Sample Syringe50 uL volume PN 410001348100 L volume PN 700002570250 L volume PN 410001347o for use with 50 L loop2023-1-3172013 Waters Corporation 182023-1-3182013 Waters Corporation 19192023-1-3192013 Waters Corporation 202023-1-3202013 Waters Corporation 212023-1-3212013 Waters Corporation

9、222023-1-3222013 Waters Corporation 232023-1-3232013 Waters Corporation 24Description P/NStatus1 L Loop430001516Optional2 L Loop430001264Optional5 L Loop430001233Optional10 L Loop430001326Standard20 L Loop430001320Optional 50 L Loop430001325Optional100 L Loop170001792(Kit)Optional250 L Loop176001780

10、(Kit)Optional2023-1-3242013 Waters Corporation 25Before running the Characterize Needle and Loop Volume function,make sure the proper volumes are configured in the Volumes Configuration menu.The above values are standard for currently shipping ACQUITY system.2023-1-3252013 Waters Corporation 26Loop

11、Volume(uL)Partial Loop w/Needle Overfill Mode Recommended sample injection volume range(uL)Partial Loop(Pressure Assist Mode)Recommended sample injection volume range(uL)Full Loop Mode Recommended sample injection volume(uL0Automatic overfill factor1uL0.2 to 0.75Not Recommended1.05.82uL0.4 to 1.5Not

12、 Recommended2.05.65uL1.0 to 3.8Not Recommended5.0510uL2.0 to 7.52.0 to 5.010.0420uL4.0 to 15.04.0 to 10.020.0350uL10.0 to 37.510.0 to 25.050.02100uL20.0 to 75.020.0 to 50.0100.02250uL40.0 to 150.040.0 to 125.0250.01.5Recommended PLNO injection volume 20%to 75%of loop volumeRecommended Partial Loop I

13、njection 20%to 50%of loop volume2023-1-3262013 Waters Corporation 27QSMQuaternary Solvent Manager SM-FTNSample Manager-Flow Through Needle CH-AHigh Temperature Column HeaterActive Pre-Heating DetectorsTUV,PDA ePDA,FLR,ELSD2023-1-3272013 Waters Corporation 28The ACQUITY H-Class System consists of the

14、 following:Quaternary Solvent Manager(QSM)Four solvent low pressure gradient mixing Supports UPLC PressuresLow gradient delay volumeSM-FTN(Sample Manager-Flow Through Needle)One injection mode-direct inject typeMuch simpler-Just specify injection volumeExcellent recovery and precisionCH-AHigh temper

15、ature column heater with active pre-heating2023-1-3282013 Waters Corporation 29Primary HeadAccumulator HeadVent Valve100ul MixerGradient Proportioning Valve PairsLow PressureManifoldDegasser ChambersSeal Wash Pump2023-1-3292013 Waters Corporation 302023-1-3302013 Waters Corporation 312023-1-3312013

16、Waters Corporation 322023-1-3322013 Waters Corporation 33Metering Syringe ValveCheck Valve and UnionInject ValveWash Solvent Inlet Line(not degassed)Leak Sensor ConnectionsSample Metering Syringe2023-1-3332013 Waters Corporation 34To Check ValveTo CH-A and ColumnStationary Side TubeFrom PumpTo Press

17、ure TransducerTo Sample Needle2023-1-3342013 Waters Corporation 35Power and Status LEDsPlate Selector SwitchRotary Tray(Plate Holder)Magnetic Door SensorPower Switch2023-1-3352013 Waters Corporation 362023-1-3362013 Waters Corporation 37Improved Door GasketAdded Tubing Gasket(top and bottom)For 3rd

18、party Mass Spec at left side ofACQUITY Stack.Allows use of existingPassive Pre-heaterNo wires to connectActive Pre-heater2023-1-3372013 Waters Corporation 382023-1-3382013 Waters Corporation 39Gold Plated Compression ScrewPEEK FerruleStainless Steel Cap NutStainless Steel Tubing(with brazed on colle

19、t)2023-1-3392013 Waters Corporation 402023-1-3402013 Waters Corporation 41Supports all current ACQUITY detectorsPDA and PDAeTUVFLRELSD2023-1-3412013 Waters Corporation 422023-1-3422013 Waters Corporation 432023-1-3432013 Waters Corporation 442023-1-3Course Name442013 Waters Corporation 452023-1-3Cou

20、rse Name452013 Waters Corporation 462023-1-3Course Name462013 Waters Corporation 472023-1-3Course Name472013 Waters Corporation 482023-1-3482013 Waters Corporation 492023-1-3Course Name492013 Waters Corporation 502023-1-3Course Name502013 Waters Corporation 51Questions?2023-1-3Course Name512013 Waters Corporation 522023-1-352