医科大学精品课件：文章构建.ppt

1、文章构建 先写摘要 列出Results大纲 在Introduction中介绍背景并引出研究目的，要求突出自己研究的 创新性和意义 在Discussion中对结果逐条分析 最后进行材料和方法的罗列 ABSTRACT Objective: Methods: Results: Conclusion: cDNA Microarray Analysis of Gene Expression in the Cerebral Cortex and Hippocampus of BALB/c Mice Subjected to Chronic Mild Stress Abstract Depressive d

2、isorders are devastating metal illness that can lead to deterioration in the social and occupational functioning of affected individuals. The etiology and pathophysiology of depression remain unknown. Present study was performed to better understand the underlying causes of depression. An experiment

3、al animal depression was induced in male BALB/c mice subjected to a chronic mild stress (CMS) procedure involving different stressor for consecutive 4 weeks. A cDNA microarray was employed to study the effects of CMS on the gene expression in cerebral cortex and hippocampus. 4-week CMS caused a sign

4、ificant reduction of 2% sucrose consumption. Morris water maze procedure showed impairment in cognitive function in stressed mice. Results of microarray showed that there were 102 and 60 genes were markedly affected by CMS treatment in cerebral cortex and hippocampus regions, respectively, including

5、 DNA damage/ repair-related enzymes, anti-oxidant enzyme, and cyclin and cyclin-dependent kinase (CDK). These findings suggest that multiple biochemical effects play an important role the etiology of depression. Results Effect of CMS on 2% Sucrose Intake Results of Morris Water Maze Test Gene Expres

6、sion Changes on Microarrays Gene Expression Changes in Cerebral Cortex Gene Expression Changes in Hippocampus Confirmation the Changes in Gene Expression by Real-Time PCR Effects of delayed motherhood on hippocampal gene expression in offspring rats ABSTRACT While many studies have examined the preg

7、nancy and health-related outcomes of delayed motherhood for women, less is known concerning the potential consequences for their children. This study aims to investigate the effect of delayed motherhood on the hippocampus at the whole genome level. SpragueDawley rat females, either at the age of 3 o

8、r 12 months, were individually housed with a randomly selected 3-month-old male. The rat whole genome expression chips were used to detect gene expression differences in the hippocampus of newborn rats. The gene expression profile was studied through gene ontology and signal pathway analyses. qRT-PC

9、R was used to determine the mRNA expression of solute carrier family 2 (SLC2A1) and S-phase kinase-associated protein 2 (SKP2). Western blot was used to detect the protein expression of SKP2. Compared to the control group, 1291 differentially expressed genes were detected, including 635 up-regulated

10、 genes and 656 down-regulated genes. These differential expressed genes were involved in 110 significant biological process and nine significant signaling pathways, in which the pathway in cancer is the most changed pathway. For SKP2 (up-regulated) and SLC2A1 (up-regulated) genes which were relevant

11、 to the pathway in cancer, qRT-PCR results were consistent with gene chip assay results. The upregulation of SKP2 was also demonstrated at protein level. In conclusion, delayed motherhood led to unique patterns of hippocampal gene expression in offspring and the newly identified genes afford a quantitative view of the changes which enable deeper insights into the molecular basis underlying the role of delayed motherhood.