综合性设计性试验内容-热激诱导的玉米幼苗耐热性观及其生理课件.ppt

1、MechanicalstimulationBiotic stressTouchWindRainWoundMechanicalstimulationBraam 2005返回Biotic stress返回Haley 1995Yahraus1995Garces 2001H2O2Ca2+NOBorsics 2001Polisensky 1996Braam 1992植物细胞中ROS产生途径Oxalate oxidaseAPXGRDecomposationCATPlantsROS ProductingROS ScavengingNADPH oxidaseCell wall peroxidaseAmine

2、oxidaseOxalate oxidasemitochondriaSODCATAPXPODGRchloroplast peroxisomeASA GSHERTocopherolNMcaretenoids氧氧化化还还原原平平衡衡6090%1040%H2O2O2.-OH.1O21mSmin4mS2mS1nS直接观察统计HeatSaltDroughtHeavy metal532nm440nm返回T r e a tm e n tInjury percentage(%)01 02 03 04 05 06 07 08 09 01 0 0Q in g 3n o n-H e a t s h o c k +H

3、 e a tH e a t s h o c k +H e a t返回T re a tm e n tSurvival percentage(%)01 02 03 04 05 06 07 0Q in g 3n o n-H e a t sh o c k +H e a tH e a t sh o c k +H e a t返回植物组织活力测定方法参考文献MDA含量测定方法参考文献VWLAA )(600532MDA content=(mmol.g-1FW)返回高O2亲和力，占耗氧量80%PPO，抗病及伤，对O2亲和力弱APX，抗逆性乙醇酸氧化酶黄素氧化酶，对温度不敏感对O2亲和力极低CAT，抗逆性POD，抗逆性SOD，抗逆性NADH/NADPHFMNUQcytbcytc1cytaa3O2交替氧化酶，抗CN-植物抗氧化酶提取和测定参考文献返回21290/240VVVtWAT CAT或APX activities=(mmol.g-1FW)21470VVVtWAT POD activities=(mmol.g-1FW)2141001.0VVtWA PPO activities=(U.g-1FW)Pro提取与测定方法参考文献VWLA 520Pro content=(mmol.g-1FW)595 nm465 nmG250G250蛋白质复合物返回调调“0”调调“100”测定测定722S使用使用