roche免疫产品原料简介课件.ppt

1、免疫产品简介picture placeholder抗体销量最好的抗体：CKMB、DD、Ferr、Insulin、E2。Roche单抗为冻干品，建议用0.9%的生理盐水溶解，溶解浓度为10mg/ml。溶解液的储存条件：1 周(+2 to+8C),12 周(-25 to-15C).请避免反复冻融。抗体肿瘤：项目项目抗体抗体批号批号备注备注CEAMABTU2 IgG MABM-TU3 IgG 11 353 713 103 10 777 498 103 鼠源单抗冻干AFPMABM-LJ738 IgG MABM-TU11 IgG 11 492 101 103 11 492 080 103 鼠源单抗冻干F

2、errMABM-3.170 IgG MABM-4.184 IgG 11 547 089 103 11 547 119 103 鼠源单抗冻干抗体内分泌：项目项目抗体抗体批号批号备注备注LHMABM-11412 IgG MABM-2.406 IgG 11 547 925103 11 547 038103 鼠源单抗冻干FSHMABM-1.303 IgG MABM-W3 IgG 11 493 540103 11 493 531103 鼠源单抗冻干HCGMABM-INN22 IgG MABM-INN2 IgG 11 812 564103 03 116 263103 鼠源单抗冻干PRLMABM-C4E4

3、IgG MABM-H12G10 IgG 11 458 701103 11 027 689103 鼠源单抗冻干E2PAKK IgG 05 344 255 103兔源多抗液体抗体甲功：项目项目抗体抗体批号批号备注备注TSH MABM-1.20 IgG MABM-A8 IgG 10 767 7781 03 11 367 978 103 鼠源单抗冻干T3PABS IgG 10 907 332 103 羊源多抗冻干T4PABS IgG 10 767 794 103 羊源多抗冻干抗体其他：项目项目抗体抗体批号批号备注备注Insulin MABM-ST3 IgG MABM-BM1 IgG 12 208 75

4、0 103 12 208 725 103 鼠源单抗冻干CK-MB MABM-7.4.5 IgG MABM-6.12.47 IgG11 719 815 103 11 719 823 103 鼠源单抗冻干D-DimerMABM-1.2.57 IgG MABM-2.1.16 IgG12 156 903 103 12 045 206 103 鼠源单抗冻干IgEMABM-323 IgG MABM-7H8 IgG 11 543 393 103 11 988 204 103 鼠源单抗冻干CRPPABS IgG 11 888 714 103羊源多抗液体酶和底物ALP底物项目项目抗体抗体批号批号备注备注ALPA

5、lkaline Phosphatase,EIA Grade10 556 602 103液体酶活3000u/mgALPAlkaline Phosphatase,recombinant,highly active03 137 031 103液体，酶活7000u/mg糖含量20-30%ALPAlkaline Phosphatase,recombinant,highlyactive,carbohydrate reduce03 535 452 103液体，酶活7000u/mg近天然，糖含量5-10%项目项目抗体抗体批号批号备注备注pNPP4-Nitrophenyl Phosphate10 004 847

6、 103结晶粉末BCIP5-Bromo-4-chloro-3-indolyl-phosphate10 760 978 103结晶粉末酶和底物POD底物项目项目抗体抗体批号批号备注备注PODPeroxidase(POD),EIA Grade10 815 462 103冻干酶活225u/mgPODPeroxidase(POD),Grade I10 121 606 103冻干酶活250u/mgPODPeroxidase(POD),Grade II11 378 783 103冻干酶活200u/mgPoly PODPoly POD,EIA Grade11 578 545 103冻干酶活600u/mg项目

7、项目抗体抗体批号批号备注备注TMB3,3,5,5-Tetramethylbenzidine10 203 700 103结晶粉末放大系统名称名称英文名称英文名称批号批号备注备注链霉亲和素 Streptavidin,recombinant11 520 679 103冻干生物素D-Biotin-N-hydroxysuccinimide ester10 734 250 103白色干粉，短链生物素D-Biotinoyl-aminocaproic acid-N-hydroxysuccinimide ester11 003 933 103白色干粉，长链链霉素包被磁珠Streptavidin Magnetic

8、 Particles11 636 502 103悬浊液磁珠直径2um左右裸珠干扰消除蛋白非特异性干扰非特异性干扰消除蛋白Bovine IgG PABB-IgG(DE)Cat.No.11293621 Interference reduction in assay system working with polyclonal bovine IgG through blocking of e.g.antibody solid phase interference Basic interference elimination.Specials:IgG fraction from bovine plas

9、ma,purified by DE chromatography with 5%aggregated IgGBSA VCat.No.10738328 Saturation of unoccupied binding sites on the solid phase Basic interference elimination.Specials:Contains no detectable IgGsSheep IgG PABS-IgG(DE)Cat.No.10717606 Interference reduction in assay system working with polyclonal

10、 sheep IgG through blocking of e.g.antibody solid phase interference Basic interference elimination.Rabbit IgG PABK-IgG(DE)Cat.No.10912280 Interference reduction in assay system working with polyclonal rabbit IgG through blocking of e.g.antibody solid phase interference Basic interference eliminatio

11、n.非特异性干扰消除蛋白BPLA I+IVBovine Plasma AlbuminCat.Nos.1172 6536 1172 6544 0353 5240 Avoidance of non-specific adsorption or antibody interactions for more complex applications with higher demands on sensitivity.Specials:High purity plus pH 4.5 treatmentType I:purity HPLC 95%,low content of steroids,lipi

12、ds and haptens Type IV:purity HPLC 95%,Type IV NEW!:purity HPLC 95%,Protease-free!2 more production steps compared to old version.Poly BSA I+IICat.Nos.11866737+11816438Due to their own polyvalence they show optimal protection against polyvalent interference(e.g.in infectious disease assays).The nega

13、tive charge also avoids hydrophobic interactions.Specials:Very efficient background signal reduction.Type I -acetylated;diameter 30 nmType II succinylated;diameter 40 nm It is recommended to combine a mono-and a polyvalent reagent!The total amount of non specific IEP is recommended to be at 2-5%.特异性

14、干扰-MAK33 家族单体 IEPsNote:The use of a framework-specific IEP is covered by Roche patent.MAK33 IgG(lyo)Cat.No 1200 941 -monoclonal IgG1 antibody with defined specificity-for test formulations working with intact IgG1-mainly for elimination of monomeric and specific interferenceIEP-FrameworkCat.No.03369

15、846 -for elimination of monomeric and highly specific Interference against the framework regions -caused by highly specific and mostly Mab-therapy-induced HAMAS -developed by comparing the amino acid-sequences of different assay antibodies -use identical immunoreactive amino acid sequences in the fr

16、amework regions of the interference eliminating reagent as in the test antibodies.-recommended for tumor marker,thyroid and cardiac assays.特异性干扰-MAK33 家族多聚体IEPs Note:The use of polymerized IEPs is covered by Roche patent.MAK33-IgG1/IgG1 Poly(liquid)Cat.No 1939 661 polymerized version of MAK33(DSS li

17、nker chemistry)for test formulations working with intact IgG1 higher efficiency concerning HAMA interference type 1 (spontaneous HAMAs)mainly for elimination of polymeric and more unspecific interference.MAK33-IgG1/Fab1 Poly(lyo)Cat.No 1368 338 recommended for test systems working with Fab conjugate

18、s comparable characteristics as the a.m.MAK33-IgG/IgG1 poly in addition it covers Fab Neo-epitopes mainly for elimination of polymeric and more unspecific interference.Poly MAK IgG2b/Fab2a(lyo)Cat.No 1355 830 recommended for test systems working with IgG 2a and/or 2b mainly for elimination of polyme

19、ric and more unspecific interferenceMAK33 家族选择推荐Assay IEPIgG1-IgG1 assay:MAK33(60g/ml)MAK33 IgG1/IgG1 poly(40g/ml)IgG1-Fab1 assay:MAK33(60g/ml)+MAK33 IgG1/Fab1 poly(40g/ml)IgG1-IgG1 highly interfered assay:MAK33(60g/ml)MAK33 IgG1/IgG1 poly(30g/ml)IEP-Framework(30g/ml)The amounts are EXAMPLES for liquid assay formulations and stand for g reagent/ml reagent buffer.The final amount can vary within a broad range.Most important is the ratio between the single reagents.We Innovate Healthcare