医学遗传学-16肿瘤遗传学eng-v课件.ppt

1、Medical Genetics G GMedical GeneticsThe ancient Greeks believed that cancer was caused by too much body fluid they called black bile.Medical GeneticsDoctors in the seventeenth and eighteenth centuries suggested that parasites caused cancer.Today,doctors understand more about the link between cancer

2、and genetics.Medical GeneticsViruses,ultraviolet(UV)radiation,and chemicals can all damage genes in the human body.If particular genes are affected,a person can develop cancer.Understanding how genes cause cancer,though,first requires a basic understanding of several genetic terms and concepts.Medic

3、al GeneticsCancer is a very common disease,affecting about 1 in 3 individuals,and about half the people that contract cancer will die as a direct result of their disease.Medical Genetics For the most part,cancer arises from a single cell,that is,cancer is a clonal disease.The average human being con

4、tains about 1014 cells(i.e.,100,000,000,000,000 cells),any one of which could,in principle,become a cancer cell,if it acquired the right sort of mutations while it still had the potential to proliferate.Medical Genetics Therefore,the cancer cell arises and progresses once out of a possible 1014 cell

5、ular targets.That only happens in 1 in 3 people.Even then it usually takes 60 or 70 years to occur.Medical GeneticsMedical GeneticsTumors are hereditary Hereditary retinoblastoma is an autosomal dominant trait in which susceptibility to retinoblastoma is inherited.This is an unusual dominant trait i

6、n that a mutation in one RB gene is not sufficient to cause symptoms,but mutations in the second allele often arise during development.Medical GeneticsMedical Genetics Offspring have a 50%chance of receiving the mutant gene from a heterozygous parent,and 90%of carriers will develop retinoblastoma,us

7、ually in both eyes.The hereditary form is also associated with a high risk for other cancers especially of the bone and fibrous tissues(osteosarcomas and fibrosarcoma.Medical Genetics Sporadic retinoblastoma is a trait in which the affected individual has not inherited any mutant alleles of the reti

8、noblastoma gene.Medical Genetics The mutations occur after birth and result in tumor formation.Tumors usually develop in only one eye and patients are not at high risk for other cancers.Both alleles need to be mutated in a single cell,and that is why this form typically occurs only in one eye.Medica

9、l GeneticsMedical GeneticsChromosome and tumors Detailed studies of the Philadelphia chromosome show that most of chromosome 22 has been translocated onto the long arm of chromosome 9.In addition,the small distal portion of the short arm of chromosome 9 is translocated to chromosome 22.This transloc

10、ation,which is found only in tumor cells,indicates that a patient has chronic myelogenous leukemia(CML).In CML,the cells that produce blood cells for the body(the hematopoietic cells)grow uncontrollably,leading to cancer.Medical GeneticsMedical GeneticsMedical Genetics The connection between this ch

11、romosomal abnormality and CML was clarified by studying the genes located on the chromosomes at the sites of the translocation breakpoints.Medical Genetics In one of the translocated chromosomes,part of a gene called abl is moved from its normal location on chromosome 9 to a new location on chromoso

12、me 22.This breakage and reattachment leads to an altered abl gene.The protein produced from the mutant abl gene functions improperly,leading to CML.Medical Genetics Oncogenes are mutated forms of genes that cause normal cells to grow out of control and become cancer cells.They are mutations of certa

13、in normal genes of the cell called proto-oncogenes.Medical Genetics Proto-oncogenes are the genes that normally control how often a cell divides and the degree to which it differentiates(or specializes).When a proto-oncogene mutates(changes)into an oncogene,it becomes permanently turned on or activa

14、ted when it is not supposed to be.When this occurs,the cell divides too quickly,which can lead to cancer.Medical Genetics It may be helpful to think of a cell as a car.For it to work properly,there need to be ways to control how fast it goes.A proto-oncogene normally functions in a way that is simil

15、ar to a gas pedal-it helps the cell grow and divide.An oncogene could be compared to a gas pedal that is stuck down,which causes the cell to divide out of control.Medical Genetics The pathway for normal cell growth starts with growth factor,which locks onto a growth factor receptor.The signal from t

16、he receptor is sent through a signal transducer.A transcription factor is produced,which causes the cell to begin dividing.If any abnormality is detected,the cell is made to commit suicide by a programmed cell death regulator.Medical Genetics More than 100 oncogenes are now recognized,and undoubtedl

17、y more will be discovered in the future.Scientists have divided oncogenes into the 5 different classes.Medical GeneticsGrowth factors These oncogenes produce factors that stimulate cells to grow.The best known of these is called sis.It leads to the overproduction of a protein called platelet-derived

18、 growth factor,which stimulates cells to grow.Medical GeneticsGrowth factor receptors These are normally turned on or off by growth factors.When they are on,they stimulate the cell to grow.Certain mutations in the genes that produce these cause them to always be on.In other cases,the genes are ampli

19、fied.Medical Genetics This means that instead of the usual 2 copies of the gene,there may be several extras,resulting in too many growth factor receptor molecules.As a result,the cells become overly sensitive to growth-promoting signals.Medical Genetics The best known examples of growth factor recep

20、tor gene amplification are erb B and erb B-2.These are sometimes known as epidermal growth factor receptor and HER2/neu.HER2/neu gene amplification is an important abnormality seen in about one third of breast cancers.Both of these oncogenes are targets of newly developed anti-cancer treatments.Medi

21、cal GeneticsSignal transducers These are the intermediate pathways between the growth factor receptor and the cell nucleus where the signal is received.Like growth factor receptors,these can be turned on or off.When they are abnormal in cancer cells,they are turned on.Medical GeneticsTranscription f

22、actors These are the final molecules in the chain that tell the cell to divide.These molecules act on the DNA and control which genes are active in producing RNA and protein.Medical Genetics The best known of these is called myc.In lung cancer,leukemia,lymphoma,and a number of other cancer types,myc

23、 is often overly activated and stimulates cell division.Medical Genetics Two well known signal transducers are abl and ras.Abl is activated in chronic myelocytic leukemia and is the target of the most successful drug for this disease,imatinib or Gleevec.Abnormalities of ras are found in many cancers

24、.Medical GeneticsProgrammed cell death regulators These molecules prevent a cell from committing suicide when it becomes abnormal.When these genes are overactive they prevent the cell from going through the suicide process.Medical Genetics This leads to an overgrowth of abnormal cells,which can then

25、 become cancerous.The most well described one is called bcl-2.It is often activated in lymphoma cells.Medical Genetics Tumor suppressor genes are normal genes that slow down cell division,repair DNA mistakes,and tell cells when to die(a process known as apoptosis or programmed cell death).Medical Ge

26、netics When tumor suppressor genes do work properly,cells can grow out of control,which can lead to cancer.About 30 tumor suppressor genes have been identified,including p53,BRCA1,BRCA2,APC,and RB1.Some of these will be described in more detail later on.Medical Genetics A tumor suppressor gene is li

27、ke the brake pedal on a car it normally keeps the cell from dividing too quickly just as a brake keeps a car from going too fast.When something goes wrong with the gene,such as a mutation,cell division can get out of control.Medical Genetics An important difference between oncogenes and tumor suppre

28、ssor genes is that oncogenes result from the activation(turning on)of proto-oncogenes,but tumor suppressor genes cause cancer when they are inactivated(turned off).Medical Genetics Another major difference is that while the overwhelming majority of oncogenes develop from mutations in normal genes(pr

29、oto-oncogenes)during the life of the individual(acquired mutations),abnormalities of tumor suppressor genes can be inherited as well as acquired.Medical GeneticsTypes of Tumor Suppressor Genes Genes that control cell divisionGenes that repair DNA Cell suicide genes Medical GeneticsGenes that control

30、 cell division Some tumor suppressor genes help control cell growth and reproduction.The RB1(retinoblastoma)gene is an example of such a gene.Abnormalities of the RB1 gene can lead to a type of eye cancer(retinoblastoma)in infants,as well as to other cancers.Medical GeneticsGenes that repair DNA A s

31、econd group of tumor suppressor genes is responsible for repairing DNA damage.Every time a cell prepares to divide into 2 new cells,it must duplicate its DNA.Medical Genetics This process is not perfect,and copying errors sometimes occur.Fortunately,cells have DNA repair genes,which make proteins th

32、at proofread DNA.But if the genes responsible for the repair are faulty,then the DNA can develop abnormalities that may lead to cancer.Medical Genetics When DNA repair genes do work,mutations can slip by,allowing oncogenes and abnormal tumor suppressor genes to be produced.The genes responsible for

33、HNPCC(hereditary nonpolyposis colon cancer)are examples of DNA repair gene defects.When these genes do not repair the errors in DNA,HNPCC can result.HNPCC accounts for up to 5%of all colon cancers and some endometrial cancers.Medical GeneticsCell suicide genes If there is too much damage to a cell D

34、NA to be fixed by the DNA repair genes,the p53 tumor suppressor gene is responsible for destroying the cell by a process sometimes described as cell suicide.Medical Genetics Other names for this process are programmed cell death or apoptosis.If the p53 gene is not working properly,cells with DNA dam

35、age that has not been repaired continue to grow and can eventually become cancerous.Medical Genetics Abnormalities of the p53 gene are sometimes inherited,such as in the Li-Fraumeni syndrome(LFS).People with LFS have a higher risk for developing a number of cancers,including soft-tissue and bone sar

36、comas,brain tumors,breast cancer,adrenal gland cancer,and leukemia.Medical Genetics Many sporadic(not inherited)cancers such as lung cancers,colon cancers,breast cancers as well as others often have mutated p53 genes within the tumor.Medical GeneticsInherited Abnormalities of Tumor Suppressor Genes

37、Inherited abnormalities of tumor suppressor genes have been found in several cancers that tend to run in families.Medical Genetics In addition to mutations in p53,RB1,and the genes involved in HNPCC,several other mutations in tumor suppressor genes can be inherited.Medical Genetics A defective APC g

38、ene causes familial polyposis,a condition in which people develop hundreds or thousands of colon polyps,some of which may eventually acquire several sporadic mutations and turn into colon cancer.Medical Genetics Abnormalities of the BRCA genes account for 5%to 10%of breast cancers.There are also man

39、y other examples of inherited tumor suppressor gene mutations,and more are being discovered each year.Medical GeneticsNon-inherited mutations of tumor suppressor genes Mutations of tumor suppressor genes have been found in many cancers.Medical Genetics For example,abnormalities of the p53 gene have

40、been found in over 50%of human cancers.Acquired mutations(those which happen during a person life)of the p53 gene appear to be involved in a wide range of cancers,including lung,colorectal,and breast cancer,as well as many others.Medical Genetics The p53 gene is believed to be among the most frequen

41、tly mutated genes in human cancer.However,acquired changes in many other tumor suppressor genes also contribute to the development of sporadic(not inherited)cancers.Medical GeneticsInherited cancer Abnormal gene Other non-inherited cancers seen with this geneRetinoblastomaRBIMany different cancers L

42、i-Fraumeni Syndrome(sarcomas,brain tumors,leukemia)P53Many different cancersMelanomaINK4aMany different cancersColorectal cancer(due to familial polyposis)APCMost colorectal cancersColorectal cancer(without polyposis)MLH1,MSH2,or MSH6Colorectal,gastric,endometrial cancers Breast and/or ovarianBRCA1,

43、BRCA2Only rare ovarian cancersWilms TumorWTIWilms tumorsNerve tumors,including brainNF1,NF2Small numbers of colon cancers,melanomas,neuroblastomaKidney cancerVHLCertain types of kidney cancersMedical GeneticsOncogene/Tumor Suppressor GeneRelated CancersBRCA1,BRCA2Breast and ovarian cancerbcr-ablChro

44、nic myelogenous leukemiabcl-2B-cell lymphomaHER2/neu(erbB-2)Breast cancer,ovarian cancer,othersN-mycNeuroblastomaEWSEwing tumorC-mycBurkitt lymphoma,othersp53Brain tumors,skin cancers,lung cancer,head and neck cancers,othersMLH1,MSH2Colorectal cancersAPCColorectal cancersMedical Genetics Multi-stage

45、 carcinogenesis starts with the development of initiated cells after interactions of acarcinogenic agent with normal(target)cells.The initiated cells have the ability to clonally expand and act as precursors for additional alterations.In different model systems initiated cells have shown some of the following characteristics.1.Increased proliferative capabilities 2.Resistance to apoptotic stimuli 3.Resistance to other inducers of cell toxicity 4.Increased life-spanMedical GeneticsMedical GeneticsMedical GeneticsMedical GeneticsMedical Genetics