简介WatersUPLC故障排除专题培训课件.ppt

1、2013 Waters Corporation 1Waters UPLC Troubleshooting Training 2013 Waters Corporation 2ACQUITY UPLC Family ：UPLC & H-ClassWaters UPLC SystemUPLC Solvent Management (BSM)UPLC Sample Management (SM)Waters ACQUITY H-Class SystemQuaternary Solvent Manager (QSM) Sample Manager-Flow Through Needle (SM-FTN

2、)Waters UPLC Detector Troubleshooting TrainingWaters UPLC PDA and PDAeTUVFLRELSDOpen Discussion2013 Waters Corporation 32013 Waters Corporation 42013 Waters Corporation 5Detectors:Optical and/or Mass SpecTunable UV or Photodiode ArrayOptimized flow cell for UPLCHigh speed data samplingColumn Manager

3、:Innovative pivot designPositions column to detectorE-Cord connectionSample Manager:Low dispersion XYZZ FormatFast cycle timesLow carryoverPlates and/or vialsOptional Sample OrganizerBinary Solvent Manager:High pressure blendingBinary gradientsFour solvent choicesOn-line degassingLow dispersion desi

4、gnUPLC pressure capabilitiesSample Organizer: (option)Expands capacity (22/15/8)Shuttles plate feedHeated/chilledSystem Considerations:Small FootprintRedesigned tubing and fittingsConsolidated waste managementIntegrated system diagnosticsConnections Insight remote diagnostics2013 Waters Corporation

5、6Waste Tubing management Chromatographic solvents:oA1/A2, B1/B2Two Sample Manager wash solventsSeal Wash Common fittings and tools for usersColumn inlets/outlets and fittings oFinger tight, turn (use 1/4” wrench)Dedicated tubing3 piece compression screw and ferrule Small footprint 13” wide24.25” wit

6、h Sample Organizer2013 Waters Corporation 72013 Waters Corporation 8 15 K psi performance Accurate and precise flow rates Accurate and precise composition Low delay volume for gradient applicationso5% Ethyl AcetateToluene Chlorinated solvents: (Trichlorobenzene) 2013 Waters Corporation 142013 Waters

7、 Corporation 15 Pressure Transducer Valve Manifold Drive Gears Wash SyringesWeak Wash Strong WashMetering Syringe Drive Assembly 2013 Waters Corporation 16 Metering Syringe Valve3 Way Rotary Valve Sample Syringe50 uL volume PN 410001348100 L volume PN 700002570250 L volume PN 410001347o for use with

8、 50 L loop2013 Waters Corporation 172013 Waters Corporation 18182013 Waters Corporation 192013 Waters Corporation 202013 Waters Corporation 212013 Waters Corporation 222013 Waters Corporation 23Description P/NStatus1 L Loop430001516Optional2 L Loop430001264Optional5 L Loop430001233Optional10 L Loop4

9、30001326Standard20 L Loop430001320Optional 50 L Loop430001325Optional100 L Loop170001792(Kit) Optional250 L Loop176001780(Kit) Optional2013 Waters Corporation 24Before running the Characterize Needle and Loop Volume function, make sure the proper volumes are configured in the Volumes Configuration m

10、enu. The above values are standard for currently shipping ACQUITY system.2013 Waters Corporation 25Loop Volume (uL)Partial Loop w/Needle Overfill Mode Recommended sample injection volume range (uL)Partial Loop (Pressure Assist Mode) Recommended sample injection volume range (uL)Full Loop Mode Recomm

11、ended sample injection volume (uL0Automatic overfill factor1uL0.2 to 0.75Not Recommended1.05.82uL0.4 to 1.5Not Recommended2.05.65uL1.0 to 3.8Not Recommended5.0510uL2.0 to 7.52.0 to 5.010.0420uL4.0 to 15.04.0 to 10.020.0350uL10.0 to 37.510.0 to 25.050.02100uL20.0 to 75.020.0 to 50.0100.02250uL40.0 to

12、 150.040.0 to 125.0250.01.5Recommended PLNO injection volume 20% to 75% of loop volumeRecommended Partial Loop Injection 20% to 50% of loop volume2013 Waters Corporation 26QSMQuaternary Solvent Manager SM-FTNSample Manager-Flow Through Needle CH-AHigh Temperature Column HeaterActive Pre-Heating Dete

13、ctorsTUV, PDA ePDA, FLR, ELSD2013 Waters Corporation 27The ACQUITY H-Class System consists of the following:Quaternary Solvent Manager (QSM)Four solvent low pressure gradient mixing Supports UPLC PressuresLow gradient delay volumeSM-FTN (Sample Manager-Flow Through Needle)One injection mode- direct

14、inject typeMuch simpler- Just specify injection volumeExcellent recovery and precisionCH-AHigh temperature column heater with active pre-heating2013 Waters Corporation 28Primary HeadAccumulator HeadVent Valve100ul MixerGradient Proportioning Valve PairsLow PressureManifoldDegasser ChambersSeal Wash

15、Pump2013 Waters Corporation 292013 Waters Corporation 302013 Waters Corporation 312013 Waters Corporation 32Metering Syringe ValveCheck Valve and UnionInject ValveWash Solvent Inlet Line (not degassed)Leak Sensor ConnectionsSample Metering Syringe2013 Waters Corporation 33To Check ValveTo CH-A and C

16、olumnStationary Side TubeFrom PumpTo Pressure TransducerTo Sample Needle2013 Waters Corporation 34Power and Status LEDsPlate Selector SwitchRotary Tray(Plate Holder)Magnetic Door SensorPower Switch2013 Waters Corporation 352013 Waters Corporation 36Improved Door GasketAdded Tubing Gasket (top and bo

17、ttom)For 3rd party Mass Spec at left side ofACQUITY Stack.Allows use of existingPassive Pre-heaterNo wires to connectActive Pre-heater2013 Waters Corporation 372013 Waters Corporation 38Gold Plated Compression ScrewPEEK FerruleStainless Steel Cap NutStainless Steel Tubing (with brazed on collet)2013

18、 Waters Corporation 392013 Waters Corporation 40Supports all current ACQUITY detectorsPDA and PDAeTUVFLRELSD2013 Waters Corporation 412013 Waters Corporation 422013 Waters Corporation 432013 Waters Corporation 442013 Waters Corporation 452013 Waters Corporation 462013 Waters Corporation 472013 Waters Corporation 482013 Waters Corporation 492013 Waters Corporation 50Questions?2013 Waters Corporation 51