子宫兴奋药对离体大鼠子宫的作用 ppt课件.ppt

1、 Excitatory effect of oxytocin on uterine smooth muscle Shi-Hong Zhang （张世红）Dept of PExperimental pharmacology of reproductive system Pituitrin (垂体后叶素垂体后叶素) Oxytocin (催产素催产素) Ergot alkaloids (麦角生物碱麦角生物碱) Prostaglandins (PGs, 前列腺素前列腺素)Pituitrin(垂体后叶素垂体后叶素) oxytocin vasopressin 垂体垂体Oxytocin (OXT, 催产素催

2、产素)1. Pharmacological effects: (1) Uterus: Elevating both the frequency and force of uterine contraction, at rhythm similar to natural labor, highly dependent on estrogen level and condition of uterus. (2) Breast: milk ejection. (3) Blood vessel: vasodilation at large dose.2. Clinical uses: - to exp

3、edite delivery and induced labor; - to reduce the incidence and severity of postpartum hemorrhage.Oxytocin (OXT, 催产素催产素)3. Adverse effects: - Intrauterine asphyxia or uterine rupture (overdose) - Allergy4. Contraindications: - hypertonic dysfunction of uterus - abnormality in birth canal - abnormal

4、fetal positionOxytocin (OXT, 催产素催产素) Ergot alkaloids(麦角生物碱麦角生物碱) Ergometrine(麦角新碱麦角新碱),Ergotamine(麦角胺麦角胺), Ergotoxine(麦角毒麦角毒)1. Pharmacological effects: (1) Uterus (ergometrine): - strongly stimulating uterine smooth muscle, both the body and the cervix; ready to induce tonic contraction at larger d

5、ose . (2) Blood vessel (ergotamine): - contracting peripheral blood vessels and cerebral vessels. (3) blocking receptor (ergotoxine):2. Clinical uses: (1) Endometrorrhagia (子宫出血子宫出血), and puerperal replacement of uterus (产后子宫复位产后子宫复位) : ergometrine; (2) Migraine (偏头痛偏头痛) : ergotamine; (3) Hibernatio

6、n therapy (冬眠疗法冬眠疗法) : dihydroergotoxine + pethidine + promethazine.Ergot alkaloids(麦角生物碱麦角生物碱)3. Adverse effects: - nausea, vomiting, BP ; - allergic reaction; - prohibited in expediting delivery and induced labor.Ergot alkaloids(麦角生物碱麦角生物碱)Prostaglandins (PGs, 前列腺素前列腺素) Dinoprostone (地诺前列酮地诺前列酮, P

7、GE2) Dinoprost (地诺前列素地诺前列素, PGF2 )1. Pharmacological effect: stimulating uterine smooth muscle. 2. Clinical uses: to induce labor2. Adverse effects: GI reaction, attack of asthma (PGF2 ); glaucoma (PGE2).Objectives To observe the excitatory effect of oxytocin on uterine smooth muscles and its dose d

8、ependent manner. Methods1.Estrodiol pretreatment (0.2mg) on rats (200 g 40g) 24hr before experiment;2.Prepare uterus smooth muscle specimen.MethodsUterus of the ratMethodsUterus of the ratMethodsPerfusion systemMethods Set parameters of recording system: Input pattern: tensility Gain: 200-500 Lower

9、frequency: direct current Upper frequency: 10 Hz Sampling interval: 2 ms Preload: 1gMethods1. The specimen is stabilized in the perfusion chamber for 15-30 min until the contractive force and rhythm become regular. Record the basal contractive force and frequency (optional). 2. Add 0.02 ml, 0.04 ml,

10、 0.14 ml oxytocin (0.01U/ml) into the perfusion system. Record the maximal force and frequency (optional) of contraction at each drug concentration accordingly.Methods3. Add 0.04 ml, 0.14 ml oxytocin (0.1U/ml) into the perfusion system. Record the maximal force and frequency (optional) of contractio

11、n at each drug concentration accordingly.4. Add 0.04 ml, 0.14 ml oxytocin (1U/ml) into the perfusion system. Record the maximal force and frequency (optional) of contraction at each drug concentration accordingly.Methods5. Add 0.04 ml, 0.14 ml oxytocin (10U/ml) into the perfusion system. Record the

12、maximal force and frequency (optional) of contraction at each drug concentration accordingly.MethodsNotice:1Avoid injury and excessive stretch of uterus during the whole process of preparation.2Control the gas bubbles in a proper rate in case of impact on uterus contraction.3Record a period of basel

13、ine of contraction before drug treatment in order to compare that before and after. 4Add drug in the order as prescribed.Lab report1题目2署名: 作者和合作者姓名及单位。3结构式摘要（目的，方法，结果，结论）。4材料和方法: 主要实验材料，标本制备方法，仪器装置连接和仪器参数，实验数据记录条件，观察指标。5结果: 实验结果的文字性描述，作量效曲线。6讨论: 回答思考题。Learning issue1缩宫素对子宫的作用及特点。2为什么实验前用雌二醇处理动物？3. 缩宫素与麦角新碱对子宫平滑肌的作用特点有何不同？